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Structured Review

BioChain Institute buffer w
Buffer W, supplied by BioChain Institute, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cnm+compartmental+protein+extraction+kit/bio_rxiv__2024__12__02__626348-171-4-10
Average 93 stars, based on 1 article reviews
buffer w - by Bioz Stars, 2026-10
93/100 stars

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Membrane:

Article Title: Cell Entry of Avian Reovirus Modulated by Cell-Surface Annexin A2 and Adhesion G Protein-Coupled Receptor Latrophilin-2 Triggers Src and p38 MAPK Signaling Enhancing Caveolin-1- and Dynamin 2-Dependent Endocytosis.
Article Snippet: .. To investigate interaction of sC with AnxA2 and ADGRL2 as well as interaction of AnxA2 and ADGRL2 in the cytoplasm membrane, the plasma membrane fraction of Vero cells was isolated using a CNM compartmental protein extraction kit according to the manufacturer’s protocol (BioChain Inc., Hayward, USA). ..

Article Title: Effect of Oral Tributyrin Treatment on Lipid Mediator Profiles in Endotoxin-Induced Hepatic Injury
Article Snippet: .. Liver tissue samples were homogenized in PRO-PREP solution (iNtRON Biotechnology, Seoul, Korea) or were fractionated into cytoplasmic, nuclear, and membrane fractions using a CNM Compartmental Protein Extraction Kit (BioChain Institute, Newark, CA, USA). .. Extracted proteins (20 μg per sample) were separated by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (GE Healthcare, Chalfont St Giles, UK).

Article Title: Effect of Oral Tributyrin Treatment on Lipid Mediator Profiles in Endotoxin-Induced Hepatic Injury
Article Snippet: .. Results are expressed as the fold-difference compared with the vehicle group at 0 h. Western blot analysis Liver tissue samples were homogenized in PRO-PREP solution (iNtRON Biotechnology, Seoul, Korea) or were fractionated into cytoplasmic, nuclear, and membrane fractions using a CNM Compartmental Protein Extraction Kit (BioChain Institute, Newark, CA, USA). .. Extracted proteins (20 μg per sample) were separated by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (GE Healthcare, Chalfont St Giles, UK).

Article Title: Additional statin treatment enhances the efficacy of HER2 blockade and improves prognosis in Rac1-high/HER2-positive breast cancer.
Article Snippet: .. Subcellular fractionation Jo ur na l P re -p ro of Membrane and cytosolic fractions were isolated using a CNM compartmental protein extraction kit according to the manufacturer’s protocol (BioChain Inc, Newark, CA, USA). ..

Article Title: Cell Entry of Avian Reovirus Modulated by Cell-Surface Annexin A2 and Adhesion G Protein-Coupled Receptor Latrophilin-2 Triggers Src and p38 MAPK Signaling Enhancing Caveolin-1- and Dynamin 2-Dependent Endocytosis
Article Snippet: .. To investigate interaction of σC with AnxA2 and ADGRL2 as well as interaction of AnxA2 and ADGRL2 in the cytoplasm membrane, the plasma membrane fraction of Vero cells was isolated using a CNM compartmental protein extraction kit according to the manufacturer’s protocol (BioChain Inc., Hayward, USA). ..

Clinical Proteomics:

Article Title: Cell Entry of Avian Reovirus Modulated by Cell-Surface Annexin A2 and Adhesion G Protein-Coupled Receptor Latrophilin-2 Triggers Src and p38 MAPK Signaling Enhancing Caveolin-1- and Dynamin 2-Dependent Endocytosis.
Article Snippet: .. To investigate interaction of sC with AnxA2 and ADGRL2 as well as interaction of AnxA2 and ADGRL2 in the cytoplasm membrane, the plasma membrane fraction of Vero cells was isolated using a CNM compartmental protein extraction kit according to the manufacturer’s protocol (BioChain Inc., Hayward, USA). ..

Article Title: Cell Entry of Avian Reovirus Modulated by Cell-Surface Annexin A2 and Adhesion G Protein-Coupled Receptor Latrophilin-2 Triggers Src and p38 MAPK Signaling Enhancing Caveolin-1- and Dynamin 2-Dependent Endocytosis
Article Snippet: .. To investigate interaction of σC with AnxA2 and ADGRL2 as well as interaction of AnxA2 and ADGRL2 in the cytoplasm membrane, the plasma membrane fraction of Vero cells was isolated using a CNM compartmental protein extraction kit according to the manufacturer’s protocol (BioChain Inc., Hayward, USA). ..

Isolation:

Article Title: Cell Entry of Avian Reovirus Modulated by Cell-Surface Annexin A2 and Adhesion G Protein-Coupled Receptor Latrophilin-2 Triggers Src and p38 MAPK Signaling Enhancing Caveolin-1- and Dynamin 2-Dependent Endocytosis.
Article Snippet: .. To investigate interaction of sC with AnxA2 and ADGRL2 as well as interaction of AnxA2 and ADGRL2 in the cytoplasm membrane, the plasma membrane fraction of Vero cells was isolated using a CNM compartmental protein extraction kit according to the manufacturer’s protocol (BioChain Inc., Hayward, USA). ..

Article Title: AMPK-related protein kinase ARK5 regulates subcellular localization of RNA-binding protein hnRNP A1 during hypertonic stress
Article Snippet: .. Nuclear and cytoplasmic fractions were isolated using a CNM compartmental protein extraction kit according to the manufacturer’s protocol (BioChain Inc). .. U2OS cells were transiently transfected with the desired plasmids for 24 h as described previously; the cells were rinsed with ice-cold PBS twice, then scraped from the wells, recovered in 1 ml of PBS, and transferred to an Eppendorf 1.5 ml tube and pelleted.

Article Title: Additional statin treatment enhances the efficacy of HER2 blockade and improves prognosis in Rac1-high/HER2-positive breast cancer.
Article Snippet: .. Subcellular fractionation Jo ur na l P re -p ro of Membrane and cytosolic fractions were isolated using a CNM compartmental protein extraction kit according to the manufacturer’s protocol (BioChain Inc, Newark, CA, USA). ..

Article Title: Cell Entry of Avian Reovirus Modulated by Cell-Surface Annexin A2 and Adhesion G Protein-Coupled Receptor Latrophilin-2 Triggers Src and p38 MAPK Signaling Enhancing Caveolin-1- and Dynamin 2-Dependent Endocytosis
Article Snippet: .. To investigate interaction of σC with AnxA2 and ADGRL2 as well as interaction of AnxA2 and ADGRL2 in the cytoplasm membrane, the plasma membrane fraction of Vero cells was isolated using a CNM compartmental protein extraction kit according to the manufacturer’s protocol (BioChain Inc., Hayward, USA). ..

Protein Extraction:

Article Title: Cell Entry of Avian Reovirus Modulated by Cell-Surface Annexin A2 and Adhesion G Protein-Coupled Receptor Latrophilin-2 Triggers Src and p38 MAPK Signaling Enhancing Caveolin-1- and Dynamin 2-Dependent Endocytosis.
Article Snippet: .. To investigate interaction of sC with AnxA2 and ADGRL2 as well as interaction of AnxA2 and ADGRL2 in the cytoplasm membrane, the plasma membrane fraction of Vero cells was isolated using a CNM compartmental protein extraction kit according to the manufacturer’s protocol (BioChain Inc., Hayward, USA). ..

Article Title: AMPK-related protein kinase ARK5 regulates subcellular localization of RNA-binding protein hnRNP A1 during hypertonic stress
Article Snippet: .. Nuclear and cytoplasmic fractions were isolated using a CNM compartmental protein extraction kit according to the manufacturer’s protocol (BioChain Inc). .. U2OS cells were transiently transfected with the desired plasmids for 24 h as described previously; the cells were rinsed with ice-cold PBS twice, then scraped from the wells, recovered in 1 ml of PBS, and transferred to an Eppendorf 1.5 ml tube and pelleted.

Article Title: Effect of Oral Tributyrin Treatment on Lipid Mediator Profiles in Endotoxin-Induced Hepatic Injury
Article Snippet: .. Liver tissue samples were homogenized in PRO-PREP solution (iNtRON Biotechnology, Seoul, Korea) or were fractionated into cytoplasmic, nuclear, and membrane fractions using a CNM Compartmental Protein Extraction Kit (BioChain Institute, Newark, CA, USA). .. Extracted proteins (20 μg per sample) were separated by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (GE Healthcare, Chalfont St Giles, UK).

Article Title: Effect of Oral Tributyrin Treatment on Lipid Mediator Profiles in Endotoxin-Induced Hepatic Injury
Article Snippet: .. Results are expressed as the fold-difference compared with the vehicle group at 0 h. Western blot analysis Liver tissue samples were homogenized in PRO-PREP solution (iNtRON Biotechnology, Seoul, Korea) or were fractionated into cytoplasmic, nuclear, and membrane fractions using a CNM Compartmental Protein Extraction Kit (BioChain Institute, Newark, CA, USA). .. Extracted proteins (20 μg per sample) were separated by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (GE Healthcare, Chalfont St Giles, UK).

Article Title: Additional statin treatment enhances the efficacy of HER2 blockade and improves prognosis in Rac1-high/HER2-positive breast cancer.
Article Snippet: .. Subcellular fractionation Jo ur na l P re -p ro of Membrane and cytosolic fractions were isolated using a CNM compartmental protein extraction kit according to the manufacturer’s protocol (BioChain Inc, Newark, CA, USA). ..

Article Title: Aspirin and 5-Aminoimidazole-4-carboxamide Riboside Attenuate Bovine Ephemeral Fever Virus Replication by Inhibiting BEFV-Induced Autophagy
Article Snippet: MDBK cells were infected with BEFV at an MOI of 1 and cultured for 18 h. The medium was harvested, and the PGE2 concentration in the culture medium of infected MDBK cells was determined using the Prostaglandin E2 EIA Kit (Cayman Chemical Co., Ann Arbor, USA). .. Cellular protein fractions were extracted through serial buffers in the CNM compartmental Protein Extraction Kit (Biochain Institute Inc., Hayward, USA). ..

Article Title: Cell Entry of Avian Reovirus Modulated by Cell-Surface Annexin A2 and Adhesion G Protein-Coupled Receptor Latrophilin-2 Triggers Src and p38 MAPK Signaling Enhancing Caveolin-1- and Dynamin 2-Dependent Endocytosis
Article Snippet: .. To investigate interaction of σC with AnxA2 and ADGRL2 as well as interaction of AnxA2 and ADGRL2 in the cytoplasm membrane, the plasma membrane fraction of Vero cells was isolated using a CNM compartmental protein extraction kit according to the manufacturer’s protocol (BioChain Inc., Hayward, USA). ..

Article Title: Cooperative recruitment of Arl4A and Pak1 to the plasma membrane contributes to sustained Pak1 activation for cell migration.
Article Snippet: .. Cell pellets were fractionated using the CNM Compartmental Protein Extraction Kit (Biochain, K3012010). ..

Western Blot:

Article Title: Effect of Oral Tributyrin Treatment on Lipid Mediator Profiles in Endotoxin-Induced Hepatic Injury
Article Snippet: .. Results are expressed as the fold-difference compared with the vehicle group at 0 h. Western blot analysis Liver tissue samples were homogenized in PRO-PREP solution (iNtRON Biotechnology, Seoul, Korea) or were fractionated into cytoplasmic, nuclear, and membrane fractions using a CNM Compartmental Protein Extraction Kit (BioChain Institute, Newark, CA, USA). .. Extracted proteins (20 μg per sample) were separated by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (GE Healthcare, Chalfont St Giles, UK).

Fractionation:

Article Title: Additional statin treatment enhances the efficacy of HER2 blockade and improves prognosis in Rac1-high/HER2-positive breast cancer.
Article Snippet: .. Subcellular fractionation Jo ur na l P re -p ro of Membrane and cytosolic fractions were isolated using a CNM compartmental protein extraction kit according to the manufacturer’s protocol (BioChain Inc, Newark, CA, USA). ..



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